Report an accessibility problem
Engineering  |  FURI

Toan Thai Nguyen

Hometown: Gilbert, Arizona, United States | Graduation Date: Spring 2020
Biomedical engineering

Cas9 Mediated Adenosine Transient Reporter for Editing Enrichment

Research Theme: Health
MORE: Spring 2020

CRISPR/Cas9 systems can be used to edit induced pluripotent stem cells to create nearly any genomic edit for disease-modeling. However, there is no readily available phenotype to enrich for edited cells. Cas9-Mediated Adenosine Transient Reporter for Editing Enrichment (CasMasTREE) was created to act as a fluorescent reporter for editing featuring a mCherry and green fluorescent protein separated by a stop codon. Upon successful base-editing of the stop codon, cells fluoresce red and green. By sorting for dual-positive cells, CasMasTREE produced singleplex editing up to 94% and multiplex editing up to 94% at genomic loci in HEK293T and stem cells.

FURI Symposium Totals

TotalProjects

0

FacultyMentors

0

OnlineSymposia

0

ResearchFocus Areas

0

FURIStudents

0

MOREStudents

0

KEENStudents

0

GCSPStudents

0