Michelle M. Mungaray
Hometown: Mesa, Arizona, United States
Graduation date: Spring 2021
FURI | Spring 2021
Development of a Genome Engineering Approach for the Modulation of Vascular PhenotypesAtherosclerosis is the leading cause of death for cardiovascular diseases and the phenotypic switch of vascular smooth muscle cells (VSMCs) mediated by the upregulation of KLF4 contributes to the progression of atherosclerotic lesions or plaque development. The objective of this study is to suppress KLF4 expression in VSMCs via a CRISPR interference mediated mechanism and consequently inhibit VSMC proliferation. The sgRNA sequences targeting KLF4 have been designed and synthesized, and the construction of the recombinant viral vector is in progress. Future work includes transfecting the construct with the packaging plasmid to produce viral particles and validate efficiency in mouse cells.
Mentor: Kuei-Chun (Mark) Wang
Sponsored project | Spring 2021
W. L. Gore & Associates is a uniquely creative, product leadership enterprise that has served a variety of global markets for 60 years, and provides innovative solutions that its associates stand behind. Gore established funds to support undergraduate students in the Fulton Undergraduate Research Initiative program and graduate students in the Master’s Opportunity for Research in Engineering program, and values student-driven research and developing relationships with students in the programs.