The primary objective of this research is to optimize the cell health of cyanobacterium Synechocystis sp. PCC6803 mutant lacking photosystem II used in Microbial Electro-photosynthesis (MEPS), where electrons are delivered to photosystem I via an electrode. Currently, high-intensity light exposure is suspected of affecting cell viability, and the utilization of glucose as an organic carbon source promotes glycogen storage, inhibiting the uptake of electrons. Research will focus on identifying the ideal organic carbon source to be used in culturing the Synechocystis using Joliot-type spectrometry and quantifying the impact of high-intensity light exposure on the organism’s photosystem I using flow cytometry.
Utilizing Acetate as a Carbon Source for Synechocystis sp. PCC6803 Mutant for Microbial Electro-Photosynthesis
The primary objective of this research is to study the effectiveness of utilizing acetate as a carbon source in the culturing of cyanobacterium Synechocystis sp. PCC6803 mutant lacking photosystem II. This organism is used in Microbial Electro-Photosynthesis (MEPS), where electrons are delivered to photosystem I via an electrode. Currently, glucose is used as a carbon source; this decreases the rate of reduction of photosystem I by allowing the Synechocystis to store glycogen from glucose during starvation periods, inhibiting the uptake of electrons. The effectiveness of acetate as a carbon source will be tested by measuring electron uptake of photosystem I using a Joliot-type spectrometer.