Anna Michelle Mangus

Chemical engineering

Hometown: Glendale, Arizona, United States

Graduation date: Spring 2022

Anna Mangus
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MORE | Spring 2022

Cryogenic Electron Tomography and 3D Reconstructions of the Intracellular Extracellular Nanowire Interface of Geobacter Sulfurreducens

Geobacter sulfurreducens are bacteria that use cellular respiration to naturally convert carbon substrates into harvestable electricity. Important morphological information of two structures involved in this process remains to be elucidated: conductive protein filaments, called microbial nanowires, and intracytoplasmic membranes (ICMs). In this work, cryo-electron tomography (cryo-ET), transmission electron microscopy (TEM) and confocal microscopy were used to image and model G. sulfurreducens nanowires and ICMs. These images reveal that nanowires appear to extend past the outer membrane and grow preferentially towards the anode, and that ICMs are induced more frequently when G. sulfurreducens is grown with insoluble solid-surface electron acceptors.

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Additional projects from this student

Optimizing cyanobacteria cell health will allow for an efficient photosynthetic-based biofuel production system.

Mentor:

  • FURI
  • Spring 2020

Studying carbon sources used in culturing cyanobacteria allows for the function of a photosynthetic-based biofuel production system.

Mentor:

  • FURI
  • Fall 2019

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