Juhi Khandelwal
Chemical engineering
Hometown: Chandler, Arizona, United States
Graduation date: Spring 2025
Additional details: Barrett, The Honors College
FURI | Fall 2022
Engineering Corynebacterium glutamicum with Malonate Pathway for Optimizing Flavonoid Production
This project seeks to address the question: What is the effect of expressing a malonate pathway in C. glutamicum strains on naringenin output? Findings suggest the optimal MatC induction with MatB is between 100 µM to 1 mM, and ideal MatC induction with aTc is between 10–50 ng/mL. Furthermore, low product titer and yield suggested that the naringenin pathway expression may be unoptimized, so RT-PCRs were run to quantify RNA transcripts of the pathway genes. Higher MatB synthase and MatC protein transporter expression levels result in greater conversion of Malonyl-coA to Naringenin. Naringenin can be used as a supplement for farm animals, enabling them to produce healthier offspring and more nutritional products for human consumption at an accelerated rate to match the growing global demand for food.
Mentor: Arul Varman
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