The standard of care for Glioblastoma (GBM) is surgical resection however, tumors reoccur within a few centimeters from the resection cavity behind an intact blood-brain-barrier (BBB). This project examined the spatiotemporal disruption of the BBB by surgical resection to deliver nanoparticles (NPs) to the peri-resection tissue (PRT). When NPs were administered immediately after surgery, they reached PRT; when administered two hours after surgery, they reached distant tissue. This work provides insight into treating GBM’s invasive tumor cells. Future studies will use immunohistochemistry to investigate the infiltration of inflammatory cells to identify possible mechanisms for NP accumulation.