The research objective is to fabricate tunable electrospun nanoscaffolds and analyze their properties to determine if it is feasible to design an implantable 3D porous substrate to induce tissue regeneration for a specific individual in vivo. A polymer solution is propelled over an electric field under set conditions to form electrospun nanoscale fibers. Tensile testing and microscopy characterize key nanoscaffold properties for tissue regeneration. This research is important to clinical applications when directing the differentiation of cells in new tissue for patients. Future work includes the use of atomic force microscopy to determine individual fibers’ nanomechanical properties.