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Developing Poly-catechols using Engineered Strains of Ecoli

This project aims to develop a microbial approach to produce a variety of poly-catechols or bio-plastics using E.coli generated CotA laccase enzyme and to analyze the properties of these bio-plastics. The aromatic substrates will be processed through specific E.coli laccase enzymes and the polymer product will be analyzed with Gel permeation chromatography. Producing these alternate

Engineering of High Yield Production of L-Serine in Cyanobacteria

Cyanobacteria have the potential to efficiently produce L-serine, an industrially important amino acid, directly from CO2 and sunlight, which is a more sustainable and cheaper source of energy as compared to current methods. The research aims to engineer a strain of cyanobacteria that increases L-serine production by mutating regulatory mechanisms that natively inhibit its production,

Strain and Process Development for Detoxifying Biomass

Furfural (2-furaldehyde) is a toxic by-product of lignocellulose, an accessible biomass, and reduces the bioprocess specific growth rates, product yields, and productivities of fermenting bacteria and yeast. C. basilensis, a soil bacterium, is capable of growing on furfural as its sole carbon and energy source. Through adaptive learning evolution and UV mutagenesis, a mutant strain