Skip to Content

Human Neural Progenitor Cell Transplantation Sustainment and Maturation with Immunodeficient Mice

This project addresses the feasibility of human neural progenitor cell (hNPC) transplantation into the striatum of immunodeficient mice. Injections of hNPCs and hNPCs encapsulated in a hyaluronic acid-laminin based hydrogel (HA-Lm gel) were performed. Immunohistochemistry (IHC) was performed 7 and 14 days post-injection for key cell lineage specific markers. These markers will allow for quantitative

Generation of an Inducible CRISPR/dCas9-KRAB System to Modulate Gene Expression

Current in-vitro Alzheimer’s disease models fail to recapitulate the critical risk factor of age because they yield immature neuronal populations that exhibit gene expression more closely resembling that of fetal brain tissue rather than adult tissue. This project aims to develop an inducible CRISPR/dCas9-KRAB system that will repress the pluripotency marker OCT4 to lead to

Large Scale Expansion and Differentiation of Pluripotent Stem Cell-Derived Neural Progenitor Cells from Amyotrophic Lateral Sclerosis Patients

Amyotrophic Lateral Sclerosis (ALS) is an aggressive neurodegenerative disease that currently presents a significant therapeutic challenge. Human pluripotent stem cell (hPSC) derived neural progenitor cells (hNPCs) offer a potential solution. HNPCs are capable of self-renewing and differentiating into multiple cell types of the central nervous system. Previous work in the lab developed a rationally designed