David Brafman

A Comparison of CRISPR/Cas-Derived Genome Editing Methods for hPSCs

Studying the benefits of alternative CRISPR systems will allow for the optimization of gene editing project designs.

Program: FURI

Performing Phenotypical Studies For An Adaptable Multi-Modality pH-Sensitive Nanoprobe for Real-Time Monitoring of Engineered Cells and Tissues

Studying noninvasive cell and tissue monitoring nanoprobes will facilitate their commercial application to improve drug screening and production.

Program: MORE

Creation of AD-Relevant Isogenic Lines with CasMasTREE

The creation of AD-relevant isogenic lines through gene editing methods can lead to the cure/treatment of Alzheimer's disease.

Program: FURI

Adaptable Multi-Modality Nanoprobes for Non-Invasive Real-Time Monitoring of Engineered Cells and Tissues

Accurate assessment of cell and tissue viability in biomanufacturing will improve research in disease modelling, drug screening and health.

Program: FURI

A Comparison of CRISPR/Cas-Derived Genome Editing Methods for hPSCs

Studying the characteristics of multiple gene editing systems will establish the optimal workflow for editing stem cells.

Program: FURI

Verification of Ratiometric pH Sensing PHPMA-Derived Polymer in Human Neuronal Tissue Models.

Characterizing the effect of a ratiometric fluorescent pH sensing nanoprobe in different tissue culture models will help scientists better assess cellular microenvironments.

Program: FURI

Cas9 Mediated Adenosine Transient Reporter for Editing Enrichment

Enriching for edited stem cells using gene editing tools can generate disease-relevant stem cell lines for disease modeling.

Program: MORE

Investigating the Role of SP5 in WNT Signaling Using an In-Vitro Model of Neural Patterning

Investigating the role of SP5 in WNT signaling will help better understand the mechanisms underlying WNT-related human defects.

Program: GCSP

Modeling Carriers and Biological Specimen Utility of a Molecular Diagnostic Biosensor for Navajo Neurohepatopathy

Diagnosing Navajo neurohepatopathy will help Phoenix Children’s Hospital better treat patients and potential parents carrying the disease.

Program: FURI

Development of Flourcent Siloxane Nano-probes as an In-Line Assay of Cell and Tissue Function

Developing and optimizing fluorescent nanoprobes to assess the functionality of cell culture and tissue live for better disease models.

Program: FURI

Development and Assessment of a Diagnostic Tool to Detect and Genotype Navajo Neurohepatopathy

Designing a real-time diagnostic tool for detection and genotyping of Navajo neurohepatopathy will improve treatment cost and time.

Program: FURI

Utilizing Siloxane-Based Nanoprobes for the In-Line Monitoring of Neural Progenitor Cells In-Vitro

Developing a live cell labeling method will help analyze the effectiveness of cell therapies used for Alzheimer’s disease.

Program: FURI

Applying Developmental Biology Principles to Probe the Mechanisms of Selective Cell Vulnerability in Alzheimer’s Disease Using Human Induced Pluripotent Stem Cells

Probing the characteristics that mediate cell-selective vulnerability or resistance will help better understand Alzheimer’s disease onset and progression.

Program: MORE

Senescence Phenotype Analysis of Progerin Induced Alzheimer’s Disease Neural Progenitor Cell Line Using Confocal Microscopy

Developing a human-induced pluripotent stem cell model for Alzheimer’s disease that reflects aging will help understand the underlying mechanisms of the disease.

Program: MORE

The Large Scale Expansion and Differentiation of Human Induced Pluripotent Stem-Cell Derived Neural Progenitor Cells

Program: FURI

Progerin-Induced Aging to Develop a Human-Induced Pluripotent Stem Cell Model of Alzheimer’s Disease

Program: FURI

Generation of an Inducible CRISPR/dCas9-KRAB System to Modulate Gene Expression

Program: FURI

Progerin-Induced Aging to Develop a Human-Induced Pluripotent Stem Cell Model of Alzheimer’s Disease

Program: FURI

Human Neural Progenitor Cell Transplantation Sustainment and Maturation with Immunodeficient Mice

Program: FURI

Generation of an Inducible CRISPR/dCas9-KRAB System to Modulate Gene Expression

Program: FURI

Large Scale Expansion and Differentiation of Pluripotent Stem Cell-Derived Neural Progenitor Cells from Amyotrophic Lateral Sclerosis Patients

Program: MORE